SmartSphero Plates
Cherry Biotech is the new distributor of the SmartSphero Plates by 4Dcell
Upto 5000+ reproducible organoids in a standard multiwell plate
SmartSphero Plates
Up to 5000+ reproducible organoids in a standard multiwell plate
Smartsphero plates —Easy, standardized organoids culture
The easiest way to start, scaleup and complexify your organoids culture base on anchoring point technology
- Reproducible with < 10% in organoids size variability
- No organoids lost during medium replacement
- Easy to track and image
- Compatible with all adherent cells
- Detect low secreted amount of soluble factors thanks to high number of organoids per well
Smartsphero plates —Easy, standardized organoids culture
The easiest way to start, scaleup and complexify your organoids culture base on anchoring point technology
- Reproducible in size and shape > than 10%
- No organoids lost during medium exchange
- Easy to track and image
- Compatible with all adherent cells
- Detect low secreted amount of soluble factors thanks to high number of organoids per well
SmartSphero Plates microstructured hydrogels is designed for simple and consistent spheroid formation and the hydrogel shape provides a microenvironment which enables the cells to sediment and self-aggregate within hours
Contact points enabling:
– to prevent organoids loss during medium exchange;
– to easily track organoids along the course of the experiments
Highlights :
- compatible with immortalised, primary, dissociated and stem cells
- work with cohesive and non-cohesive cells
- suited for various set of organs : lung, breast, ovary, adipose tissues, kidney, cervix, liver and more.
- maintain organoids culture for short-term (24-48h) and long-term (up to more than a month)
Supported Applications
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Have a specific application in 3D cell culture?
Our SmartSphero Plates is highly customizable. Talk to us to receive a free consultation on your project.
SPECIFICATIONS
STANDARD MULTIWELL PLATES WITH MICROWELLS FOR SPHEROIDS’ ASSEMBLY:
6, 12 and 24 well plates, with micro-well size of 14 mm or 10 mm
Each plates’ well has 19, 55 or 91 individual microwells where spheroids are cultured
The microstructured hydrogel are made of biocompatible polyethylene glycol
Optionally the microwells can be coated with an ECM matrix to increase the physio mimetism and adherence of the spheroid to the anchoring point
The gels are preserved in a water based solution to keep their mechanical and optical properties
(Please allow cookies to watch the video below)
SCHEME OF THE MICROSTRUCTURED HYDROGEL:
EXAMPLE OF LOSS OF SPHEROIDS AFTER MANIPULATION (WITH AND WITHOUT ANCHORING POINTS):
PROTOCOL OVERVIEW
FAQ
Each SmartSphero Plate (SSP) comes in individual packaging
The SmartSphero Plates (SSP) are compatible with glass bottom plates for 6, 12, and 24 well formats, with micro-well sizes of 14 mm or 10 mm.
The size of the spheroids or organoids after culture typically ranges between 150 µm and 500 µm.
The SmartSphero Plates (SSP) have an expiration date of 1 month.
Store the SmartSphero Plates (SSP) sealed at room temperature.
Organoids can be collected at the end of culture by individually pipetting them or through enzymatic digestion.
The SmartSphero Plates (SSP) alone do not support vascularization. Vascularization can be achieved using the CubiX platform.
FAQ
Each SmartSphero Plate (SSP) comes in individual packaging
The SmartSphero Plates (SSP) are compatible with glass bottom plates for 6, 12, and 24 well formats, with micro-well sizes of 14 mm or 10 mm.
The size of the spheroids or organoids after culture typically ranges between 150 µm and 500 µm.
The SmartSphero Plates (SSP) have an expiration date of 1 month.
Store the SmartSphero Plates (SSP) sealed at room temperature.
Organoids can be collected at the end of culture by individually pipetting them or through enzymatic digestion.
The SmartSphero Plates (SSP) alone do not support vascularization. Vascularization can be achieved using the CubiX platform.
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