Scientific notes

3D Microphysiological System- Vascularized Tissue Constructs for Regenerative Medicine

Introduction

Human vascular microphysiological systems (MPS) represent promising three-dimensional in vitro models of normal and diseased vascular tissue. These systems, which mimic essential functional components of organs and tissues, draw on breakthroughs in tissue engineering, microfluidics, and stem cell differentiation.

Vascular models for microvasculature and medium-sized arterioles have already been established. Key vascular system functions have been replicated, and stem cells have the ability to mimic hereditary disorders and population diversity in genes that may raise an individual’s risk of cardiovascular disease. These systems can be used to assess novel treatment alternatives. Learn more in the paper featured below.

How to culture vascularized & immunocompetent 3D models in a standard Multiwell

Abstract

The authors state that “Microphysiological systems (MPSs), based on microfabrication technologies and cell culture, can faithfully recapitulate the complex physiology of various tissues. However, 3D tissues formed using MPS have limitations in size and accessibility; their use in regenerative medicine is, therefore, still challenging.

Here, an MPS-inspired scale-up vascularized engineered tissue construct that can be used in regenerative medicine is designed. Endothelial cell-laden hydrogels are sandwiched between two through-hole membranes. The microhole array in the through-hole membranes enables the molecular transport across the hydrogel layer, allowing long-term cell culture. 3D Microphysiological System Vascularized Tissue

Furthermore, the time-controlled delamination of through-hole membranes enables the harvesting of cell-cultured hydrogel constructs without damaging the capillary network. Importantly, when the tissue constructs are implanted in a mouse ischemic model, they protect against necrosis and promoted functional recovery to a greater extent than implanted cells, hydrogels, and simple gel–cell mixtures.”.

References

Bang, S., Tahk, D., Choi, Y. H., Lee, S., Lim, J., Lee, S., Kim, B., Kim, H. N., Hwang, N. S., & Jeon, N. L. (2021). 3D Microphysiological System‐Inspired Scalable Vascularized Tissue Constructs for Regenerative Medicine. Advanced Functional Materials, 2105475. https://doi.org/10.1002/adfm.202105475

Related Posts

Vascular smooth muscle-derived Trpv1+ progenitors are a source of...
Introduction Brown fat is a kind of body fat that is activated when we get cold, also known as brown adipose tissue. It generates heat to ke...
Read more
Internal-Anatomy-of-the-Heart
Heart On a Chip: State of the Art...
Why a heart on a chip? The heart is the central element of the cardiovascular system. If it is dysfunctional, other organs will not receive ...
Read more
3d-cell-culture-Cherry-Biotech-CubiX-microphysiological-system-adipocytes-diabetes-adiponectin
Probing Insulin Sensitivity with Metabolically Competent Human St...
Introduction Adipose Tissue is very versatile and essential for studies related to Obesity and Diabetes, however, the high buoyancy, fra...
Read more

get in touch

Get the best insights about Cherry Biotech by Email Let’s stay in touch!