Adipose Tissue
Human Mature Adipose Tissue Organoid
Applications
- Adipose tissue physiology and mechanisms (FRX, SIRT1, PRDM16, LXR, CPT1, CB1, GPR120, FSTL1, Adiponectin agonists, adipocytes-endothelial cells pathways, adipocytes-macrophages pathways, endocrine modulation)
- Target validation and optimization
- Complementary efficacy NID/CTA in vitro data
- Disease modeling (Lipid droplet enlargement and cellular hypertrophy)
Endpoints
- Adipocyte dysfunction biomarkers (adiponectin-to-leptin ratio, lipid droplet size…)
- Insulin resistance
- Lipid metabolism (triglycerides accumulation, glycerol release)
- Real-time monitoring of glucose metabolism
- Pro-inflammatory markers evaluation
Beyond these core endpoints:
Our platform provides on-demand sample access for a full suite of advanced analyses—including cytokine profiling, histology, multi-omics, and phenotype characterization—giving you a comprehensive understanding of your adipose tissue model from a single, integrated system.
Unique
Cherry Biotech expertise
We offer fine-tuned control of the microenvironment with our CubiX platform for improved physiological relevance, robustness and reproducibility:
- Mature human unilocular adipocytes
- A culture supported by differentiated vascular interface
- SmartSphero Plates microstructured hydrogels by 4DCell, designed to improve standardization and reproducibility of your organoids culture (< 10% size variability)
- Improved adipocytes organoid volume and viability (no necrotic core)
- Maintenance of physiological progenitor reservoir (pre-adipocytes)
- Long-term culture (> 30 days)
Why adipose tissue organoid models matter?
With the global rise of obesity and related metabolic diseases due to changes in modern lifestyle, the need for appropriate treatments is urgently needed and understanding the cell-based mechanisms of these conditions is key to developing solutions.
However, traditional 2D cell cultures often fail to obtain single lipid droplet adipocytes characteristic of white adipose tissue and do not provide the appropriate environment (extracellular matrix, endothelial cells…). These issues slow down drug discovery and limit the potential to generate accurate, human-relevant data essential for successful pharmaceutical development.
The human mature adipose tissue organoid model – an application of CubiX platform by Cherry Biotech – provides a cutting-edge 3D model to validate target and mechanism of action of drug candidates and features vascularized unilocular adipocytes derived from stromal vascular fraction
Using our CubiX perfusion platform, we offer scientists a highly relevant and scalable model to study cellular and molecular mechanisms related to obesity and metabolic diseases while reducing reliance on animal testing and supporting the 3Rs (Replacement, Reduction, and Refinement) in ethical research.
Other Organ Models
Our Key to Achieve These Models in 3D
CubiX – Microphysiological (MPS) platform is the only microenvironment controller fully compatible with live imaging. It enables mimicking physiological and pathological culture conditions in vitro.
Scalable, highly customisable and user-friendly.
download the complete applications of CubiX - Microphysiological platform
FAQ
Using the CubiX platform we can fine-tune the temperature, flow rate, shear stress and the dissolved gases (O2, CO2, N2) of the system to create the appropriate experimental conditions. Variables such as the pH, release of lactate, the O2 and the consumption of glucose can be monitored to ensure smooth experiment run.
If you would like more detailed information on the features offered by the CubiX platform, visit our page.
Yes, the CubiX perfusion platform features a microfluidic perfusion lid (MPL) adapter compatible with standard 6 and 24 multi well plate and can accommodate Transwell inserts. The design enables transforming static cell culture in standard multiwell into a perfusion dynamic system.
Our adipose tissue model reproduces key characteristics of white adipose tissue, including unilocular adipocytes that contain a single, large lipid droplet. This droplet displaces other organelles—such as the nucleus—to the periphery of the cell. In contrast, brown and beige adipocytes possess multiple smaller (multilocular) lipid droplets.
White adipose tissue primarily functions in fat/energy storage and endocrine regulation, and its dysfunction is associated with obesity and other metabolic disorders. On the other hand, brown adipose tissue’s main role is thermogenesis (heat production). (Richard et al, 2020)
Medium flow and the resulting shear stress are essential not only for the physiological alignment of endothelial cells—ensuring a proper endothelial barrier—but also for the differentiation and viability of the adipose tissue model. Through perfusion, we demonstrate improved adipocyte volume (increased lipid storage) and enhanced viability. Perfusion ensures adequate nutrient and oxygen delivery, as well as efficient waste removal, preventing the necrotic core often associated with large organoids. This method supports long-term cultures of over 30 days.
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